Advances in Clinical Chemistry, Vol. 29 by Herbert E. Spiegel (ed.)

By Herbert E. Spiegel (ed.)

The sector of medical chemistry now encompasses points of analytical chemistry, biochemistry, scientific laboratory technology, toxicology, immunology and molecular biology. This serial offers a extra finished international view of the present and destiny instructions of this increasing self-discipline. This quantity offers topical papers realted typically to the kidney, melanoma and purposes of the polymerase chain response.

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At the exon-intron boundaries the sequences of the donor and acceptor splice junctions are rather unique. The first intron contains either an unusual donor sequence-GG instead of the highly conserved GT-or an unusual acceptor sequence-GG instead of Ag. The promoter region lacks both TATA and CAT boxes. The region is rich in GC and contains eight repeats of the consensus hexanucleotide core for binding transcription factor Spl (GGGCGG). Ho and Crapo (H14) cloned full-length Mn-SOD cDNAs from libraries of human liver and U937 cells and obtained a full primary sequence.

However no data were available regarding the levels of Mn-SOD protein after TNF treatments. The development of a monoclonal antibody and the ELISA technique made it possible to determine quantitatively Mn-SOD protein levels. The ELISA was used to study changes in the immunoreactive Mn-SOD levels in TNF-resistant and -sensitive human cell lines in response to TNF. 1. Effect of TNF on the Levels of Mn-SOD and Cu,Zn-SOD in TNFResistant Cells The effect of TNF on the expression of Mn-SOD protein in TNF-resistant cells was examined.

26). This indicates that different mechanisms are operating for the release of these two enzymes. CLINICAL SIGNIFICANCE OF SOD 29 101 -4000 f 70 I I i . 600 3 25a --. -3000 j -. 2 r - v 0 M 7 400 c 200 i -2000 0 4 7- 0 I ? 0.. M. 800- 7I i + I -I a 1 f 400- 2 f y z I- 200- z v) a 0.. 0, M. 17. Typical patterns of Mn-SOD release into serum for two male patients with acute myocardial infarction. (A) A case with an unsuccessful reperfusion; (B) successful case. The mean level for normal male adults is almost 100 ng/ml.

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