By Stanley T. Crooke and Archie W. Prestayko (Eds.)
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Figures 2 and 3 show the schema suggested by Iyer and Szybalski (1963, 1964). The proposed activation is rapid and the active intermediates are unstable; therefore DNA must be present during the reduction. Activation in vivo is NADPH dependent but can be performed in a cell-free system using isolated DNA and sodium hydrosulfite. When activated, mitomycin C is a bifunctional or trifunctional alkylating agent. , 1977). Many structural analogues of mitomycin C have been synthesized in an effort to improve the therapeutic ratio.